Regulation of growth and dissemination of a human lymphoma by CD44 splice variants

Details

Serval ID
serval:BIB_013351BCF212
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulation of growth and dissemination of a human lymphoma by CD44 splice variants
Journal
Journal of Cell Science
Author(s)
Bartolazzi  A., Jackson  D., Bennett  K., Aruffo  A., Dickinson  R., Shields  J., Whittle  N., Stamenkovic  I.
ISSN
0021-9533 (Print)
Publication state
Published
Issued date
1995
Volume
108 ( Pt 4)
Pages
1723-1733
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S
Abstract
CD44 is a polymorphic cell surface glycoprotein, currently proposed to be the principal cell surface receptor for hyaluronan. However, different isoforms of CD44, expressed in human lymphoid tumor cells, appear to have distinct effects on the ability of the cells to attach to hyaluronan-coated surfaces and on their capacity to form tumors in vivo. In the present study, we address the mechanisms that may regulate CD44 isoform-dependent adhesion to hyaluronan. We use a human Burkitt lymphoma, stably transfected with six different alternatively spliced human CD44 isoforms, to determine their potential hyaluronan binding and tumor growth promoting roles. We show that transfectants expressing CD44 splice variants that contain variable exons 6-10, 7-10 and 8-10 adhere to hyaluronan-coated surfaces weakly and that corresponding tumor formation in vivo is delayed with respect to CD44-negative parental cell-derived tumors. Abundant shedding of these three isoforms may play a significant role in determining the rate of tumor development. Transfectants expressing variable exon 3, on the other hand, fail to display CD44-mediated adhesion to hyaluronan, but form bone marrow tumors rapidly following intravenous injection. These observations suggest that different mechanisms regulate CD44-mediated adhesion and tumor growth, and provide evidence that expression of exon v3 may confer novel ligand-binding properties
Keywords
Alternative Splicing/Animals/Antigens,CD/analysis/biosynthesis/Antigens,CD44/Burkitt Lymphoma/immunology/Pathology/Carrier Proteins/Cell Adhesion/Cell Division/Cell Line/Chondroitin Lyases/Exons/Humans/Hyaluronic Acid/Hyaluronoglucosaminidase/Mice/Mice,Nude/Polysaccharide-Lyases/Receptors,Cell Surface/Receptors,Lymphocyte Homing/Recombinant Fusion Proteins/Transfection/Transplantation,Heterologous/Tumor Cells,Cultured/Research
Pubmed
Web of science
Create date
29/01/2008 19:33
Last modification date
03/03/2018 13:14
Usage data