Determination of group I metabotropic glutamate receptor subtypes involved in the frequency of epileptiform activity in vitro using mGluR1 and mGluR5 mutant mice.

Details

Serval ID
serval:BIB_013029E53FA6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Determination of group I metabotropic glutamate receptor subtypes involved in the frequency of epileptiform activity in vitro using mGluR1 and mGluR5 mutant mice.
Journal
Neuropharmacology
Author(s)
Stoop R., Conquet F., Pralong E.
ISSN
0028-3908
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
44
Number
2
Pages
157-162
Language
english
Abstract
In mouse hippocampal slices, bicuculline elicited spontaneous epileptiform bursts with a duration of 200-300 ms and with a frequency of five to six events per minute. Application of group I metabotropic glutamate receptor agonist (RS)-3,5-dihydroxyphenylglycine ((RS)-DHPG) increased the burst frequency up to 300% at concentrations of 50 to 100 microM, while it decreased the burst duration below 100 ms. In slices of subtype I mGluR1 or subtype I mGluR5 knockout mice, bicuculline elicited spontaneous epileptiform bursts with similar duration and frequency as those measured in wild-type mice but without the previous effects seen following application of DHPG at concentrations up to 100 microM. Likewise, in slices of wild-type mice, preincubation with mGluR1 antagonist, 1-aminoindan-1,5-dicarboxylic acid (AIDA) or mGluR5 receptor antagonist, 2-methyl-6-(phenylethynyl)-pyridine (MPEP) blocked in both cases completely the increase in frequency following DHPG application. These findings suggest an interactive mechanism between mGluR1 and mGluR5 receptors in the modulation of epileptiform bursting activity by DHPG that could indicate a common intracellular signaling mechanism or possibly direct interaction between these two receptors.
Keywords
Action Potentials/drug effects, Animals, Benzoates/pharmacology, Bicuculline/pharmacology, Dose-Response Relationship, Drug, Drug Interactions, Epilepsy/chemically induced, Epilepsy/physiopathology, Excitatory Amino Acid Antagonists/pharmacology, Female, GABA Antagonists/pharmacology, Glycine/analogs &amp, derivatives, Glycine/pharmacology, Hippocampus/cytology, Hippocampus/drug effects, Indans/pharmacology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout/genetics, Mice, Knockout/physiology, Pyridines/pharmacology, Receptors, Metabotropic Glutamate/classification, Receptors, Metabotropic Glutamate/genetics
Pubmed
Web of science
Create date
11/03/2008 11:57
Last modification date
20/08/2019 13:23
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