Accuracy of a Single, Heparin-Calibrated Anti-Xa Assay for the Measurement of Rivaroxaban, Apixaban, and Edoxaban Drug Concentrations: A Prospective Cross-Sectional Study.

Details

Serval ID
serval:BIB_00F12CE54CC0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Accuracy of a Single, Heparin-Calibrated Anti-Xa Assay for the Measurement of Rivaroxaban, Apixaban, and Edoxaban Drug Concentrations: A Prospective Cross-Sectional Study.
Journal
Frontiers in cardiovascular medicine
Author(s)
Meihandoest T., Studt J.D., Mendez A., Alberio L., Fontana P., Wuillemin W.A., Schmidt A., Graf L., Gerber B., Amstutz U., Bovet C., Sauter T.C., Asmis L.M., Nagler M.
ISSN
2297-055X (Print)
ISSN-L
2297-055X
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
9
Pages
817826
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Applying a single anti-Xa assay, calibrated to unfractionated heparin to measure rivaroxaban, apixaban, and edoxaban would simplify laboratory procedures and save healthcare costs.
We hypothesized that a heparin-calibrated anti-Xa assay would accurately measure rivaroxaban, apixaban, and edoxaban drug concentrations and correctly predict clinically relevant drug levels.
This analysis is part of the Simple-Xa study, a prospective multicenter cross-sectional study conducted in clinical practice. Patients treated with rivaroxaban, apixaban, or edoxaban were included. Anti-Xa activity was measured using the Siemens INNOVANCE <sup>®</sup> Heparin assay. Drug concentrations were determined using ultra-high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cut-off levels were determined in a derivation dataset (50% of patients) and sensitivities and specificities were calculated in a verification dataset (50% of patients).
Overall, 845 patients were available for analysis. Correlation coefficients (r <sub>s</sub> ) between the heparin-calibrated anti-Xa assay and drug concentrations were 0.97 (95% CI 0.97, 0.98) for rivaroxaban, 0.96 (0.96, 0.97) for apixaban, and 0.96 (0.94, 0.99) for edoxaban. The area under the receiver operating characteristics curve (ROC) was 0.99 for all clinically relevant drug concentrations. In the verification dataset, the sensitivity was 94.2% (95% CI 90.8-96.6) for 30 μg L <sup>-1</sup> , 95.8% (92.4-98.0) for 50 μg L <sup>-1</sup> , and 98.7% (95.5-99.9) for 100 μg L <sup>-1</sup> . Specificities were 86.3% (79.2-91.7), 89.8% (84.5-93.7), and 88.7% (84.2-92.2), respectively.
In a large prospective study in clinical practice, a strong correlation of heparin-calibrated anti-Xa measurements with LC-MS/MS results was observed and clinically relevant drug concentrations were predicted correctly.
Keywords
anti-Xa assay, diagnostic accuracy, direct oral anticoagulants, laboratory monitoring, rivaroxaban
Pubmed
Web of science
Create date
11/04/2022 8:43
Last modification date
15/07/2022 6:36
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