ISEcp1-Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae.

Kieffer, N.; Poirel, L.; Mueller, L.; Mancini, S.; Nordmann, P.

doi:10.1128/AAC.00150-20

ISEcp1-Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae.

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Serval ID

serval:BIB_006C9C8E6CAA

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

ISEcp1-Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae.

Journal

Antimicrobial agents and chemotherapy

Author(s)

Kieffer N., Poirel L., Mueller L., Mancini S., Nordmann P.

ISSN

1098-6596 (Electronic)

ISSN-L

0066-4804

Publication state

Published

Issued date

21/04/2020

Peer-reviewed

Oui

Volume

Number

Pages

e00150-20

Language

english

Notes

Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish

Abstract

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-bla <sub>CTX-M-14b</sub> tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the fosA gene, resulting in fosfomycin resistance. This work showed the concomitant acquisition of resistance to broad-spectrum cephalosporins and fosfomycin due to a single genetic event.

Keywords

Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Cephalosporins/pharmacology, DNA Transposable Elements/genetics, Drug Resistance, Multiple, Bacterial/genetics, Fosfomycin/pharmacology, Genome, Bacterial/genetics, Humans, Klebsiella pneumoniae/drug effects, Klebsiella pneumoniae/genetics, Klebsiella pneumoniae/isolation & purification, Microbial Sensitivity Tests, Mutagenesis, Insertional/genetics, Transcription Factors/genetics, Whole Genome Sequencing, beta-Lactamases/genetics, FOS, FosA, ISEcp1, Klebsiella pneumoniae, fosfomycin

OAI-PMH

oai:serval.unil.ch:BIB_006C9C8E6CAA

DOI

10.1128/AAC.00150-20

Pubmed

32122889

Web of science

000528256200007

Open Access

Yes

Create date

05/03/2020 15:13