Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.

Details

Serval ID
serval:BIB_002FBB72C992
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.
Journal
Methods In Molecular Biology
Author(s)
Dangaj D., Scholler N.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
1319
Pages
37-49
Language
english
Abstract
B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ovarian cancer patients and we screened for functional scFvs capable to interfere with B7-H4-mediated inhibition of antitumor responses. We found one antibody binding to B7-H4 that could restore antitumor T cell responses. This chapter gives an overview of the methods we developed to isolate a functional anti-B7-H4 antibody fragment.
Keywords
Animals, Antigen Presentation, B-Lymphocytes/immunology, Female, Gene Library, Humans, Mice, Ovarian Neoplasms/blood, Ovarian Neoplasms/immunology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Single-Chain Antibodies/genetics, Single-Chain Antibodies/isolation & purification, T-Lymphocytes/immunology, V-Set Domain-Containing T-Cell Activation Inhibitor 1/metabolism, Xenograft Model Antitumor Assays
Pubmed
Create date
17/03/2016 18:55
Last modification date
20/08/2019 12:22
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